Effectiveness of the Use of Different Tannins as an Antibacterial in Escherichia Coli

Abstract

Escherichia coli are bacterium, which is needed in the digestive. However, the high increase of Escherichia coli in the digestive can cause indigestion such as diarrhea. The increased of Escherichia coli in digestion can inhibit by administering antibacterial (antiseptic is a chemical compounds used to inhibit bacterial growth). This experiment to test the effectiveness of the use of different Tannins as an antibacterial in Escherichia coli. Tannins are Phenol compounds, which have a section that acts antiseptic. In addition to synthetic Phenol, Phenol compounds also found in nature or so called Polyphenol. The materials testing Tannins derived from the leaves and bark of Psidium guajava. This experiment using a qualitative test for presence of Tannins and to identify the class of Tannins and quantitative test for analyzing function based of the phenol group. Qualitative test using indicators FeCl₃. Qualitative test with analyzing from the result of qualitative test based on the phenol group. The result showed Psidium guajava leaves produce a green color when hatching FeCl_3. This is showing that the Psidium guajava leaves are Catechol Tannins, which have two of phenol cluster. Psidium guajava barks produce a blue-black color when hatching FeCl₃. This is showing that the Psidium guajava barks are Pirogalotannin Tannins, which have three of phenol cluster. Temporary conclusion showed of difference of phenol cluster in simplicia can cause the difference of the work effectiveness as an antibacterial. Tannins class different can affect of antibacterial inhibitory in Escherichia coli.

Keywords: Escherichia coli, Psidium guajava, Tannins